Growth and Quantification of MERS‐CoV Infection
Identifieur interne : 001776 ( Main/Exploration ); précédent : 001775; suivant : 001777Growth and Quantification of MERS‐CoV Infection
Auteurs : Christopher M. Coleman ; Matthew B. FriemanSource :
- Current Protocols in Microbiology [ 1934-8525 ] ; 2015.
Descripteurs français
- KwdFr :
- ARN viral (analyse), ARN viral (isolement et purification), Animaux, Cellules Vero, Charge virale (), Coronavirus du syndrome respiratoire du Moyen-Orient (croissance et développement), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Culture virale ().
- MESH :
- analyse : ARN viral.
- croissance et développement : Coronavirus du syndrome respiratoire du Moyen-Orient.
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient.
- isolement et purification : ARN viral, Coronavirus du syndrome respiratoire du Moyen-Orient.
- Animaux, Cellules Vero, Charge virale, Culture virale.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (growth & development), Middle East Respiratory Syndrome Coronavirus (isolation & purification), RNA, Viral (analysis), RNA, Viral (isolation & purification), Vero Cells, Viral Load (methods), Virus Cultivation (methods).
- MESH :
- chemical , analysis : RNA, Viral.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- growth & development : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus, RNA, Viral.
- methods : Viral Load, Virus Cultivation.
- Animals, Chlorocebus aethiops, Vero Cells.
Abstract
Middle East respiratory syndrome coronavirus (MERS‐CoV) is an emerging highly pathogenic respiratory virus. Although MERS‐CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS‐CoV and RNA products of replication in vitro. MERS‐CoV is able to infect a range of cell types, but replicates to high titers in Vero E6 cells. Protocols for the propagation and quantification of MERS‐CoV are presented. © 2015 by John Wiley & Sons, Inc.
Url:
DOI: 10.1002/9780471729259.mc15e02s37
PubMed: 26344219
PubMed Central: 4735735
Affiliations:
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Le document en format XML
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